molecular detection of extended spectrum b-lactamase (esbl) genes blactx-m, blatem and blashv in escherichia coli isolated from water buffalo (bubalus bubalis) feces in west azerbaijan province, iran

نویسندگان

سید شهرام حسینی

دانش آموخته، دانشکده دامپزشکی دانشگاه ارومیه، ارومیه–ایران حبیب دستمالچی ساعی

گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه ارومیه، ارومیه–ایران عبدالغفار اونق

گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه ارومیه، ارومیه–ایران

چکیده

background: beta-lactamase enzymes are considered the most important factor of resistance against b-lactam antibiotics among gram-negative bacteria. in recent years, the production of extended-spectrum b-lactamases has been prevailed among bacteria, especially bacteria of animal origin, and this is important in terms of public health. objective: the prupose of this study is to evaluate the presence of extended-spectrum b-lactamase (esbl)-genes blactx-m, blatem and blashv in e. coli isolates recovered from fecal samples of apparently healthy water buffaloes (bubalus bubalis) using polymerase chain reaction. methods: in this study, 105 isolates of e. coli, which were obtained from 135 fecal samples of water buffaloes from different areas of west azerbaijan province (33 isolates from urmia, 33 isolates from khoy, 24 isolates from piranshahr and 15 isolates from miandoab), were identified using biochemical characteristics as well as 23s rrna gene amplification. then, the presence of ctx-m, tem, and shv groups of esbl genes were evaluated among the studied e. coli isolates by the pcr method. results: in the studied isolates, 47 out of 105 e. coli isolates (44.8%) contained blactx-m gene and 37 isolates (35.2%) harbored blatem gene. also, 17 isolates (16.2%) contained both blactx-m and blatem genes simultaneously. according to the results, blashv gene was not detected among the studied isolates. also, no significant difference was seen in distribution of esbl genes among the studied regions. conclusions: the results of this study indicate that water buffalo gastrointestinal e. coli is reservoir for esbls, especially ctx-m and tem types, and this should be considered in terms of public health and the transfer of resistance genes to pathogenic bacteria.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fecal carriage of Escherichia coli harboring extended-spectrum beta-lactamase (ESBL) genes by sheep and broilers in Urmia region, Iran

Background: There is a growing concern on the impact of the presence of extended-spectrum β-lactamase (ESBL) producing Escherichia coli isolated from animals on public health. OBJECTIVES: The aim of this study was to investigate the presence of three classes of ESBL genes in E. coli isolates from sheep and broilers at a slaughter in Urmia region, Iran. METHODS: A total of 111 E. coli isolates w...

متن کامل

Prevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran

Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods ...

متن کامل

Distribution of blaTEM, blaSHV, and blaCTX-M genes among ESBL-producing P. aeruginosa isolated from Qazvin and Tehran hospitals, Iran

INTRODUCTION Pseudomonas aeruginosa is as an important opportunistic human pathogen, which is associated with several clinical infections that are usually difficult to treat because of resistance to multiple antimicrobials. The production of extendedspectrum ß-lactamases (ESBLs) is an important mechanism of ß-lactam resistance. The aims of this study were to determine the prevalence of ESBLs, a...

متن کامل

Prevalence of Extended-spectrum Beta-lactamases (ESBL) Types blaTEM and blaSHV in Klebsiella pneumoniae Strains Isolated from Clinical Samples by PCR in Miandoab, West Azerbaijan

Background and Objective: Beta-lactamases are the most important factors in the resistance to beta-lactam antibiotics among gram-negative bacteria, especially Klebsiella pneumoniae. Nowadays, the prevalence of infections caused by extended-spectrum β-lactamases (ESBLs)-producing K. pneumoniae is increasing, as one of the emerging health problems throughout the world. This study aimed to investi...

متن کامل

Comparative possession of Shiga toxin, intimin, enterohaemolysin and major extended spectrum beta lactamase (ESBL) genes in Escherichia coli isolated from backyard and farmed poultry

The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them posse...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
تحقیقات دامپزشکی

جلد ۶۹، شماره ۳، صفحات ۲۰۳-۲۱۲

کلمات کلیدی
background: beta lactamase enzymes are considered the most important factor of resistance against b lactam antibiotics among gram negative bacteria. in recent years the production of extended spectrum b lactamases has been prevailed among bacteria especially bacteria of animal origin and this is important in terms of public health. objective: the prupose of this study is to evaluate the presence of extended spectrum b lactamase (esbl) genes blactx m blatem and blashv in e. coli isolates recovered from fecal samples of apparently healthy water buffaloes (bubalus bubalis) using polymerase chain reaction. methods: in this study 105 isolates of e. coli which were obtained from 135 fecal samples of water buffaloes from different areas of west azerbaijan province (33 isolates from urmia 33 isolates from khoy 24 isolates from piranshahr and 15 isolates from miandoab) were identified using biochemical characteristics as well as 23s rrna gene amplification. then the presence of ctx m tem and shv groups of esbl genes were evaluated among the studied e. coli isolates by the pcr method. results: in the studied isolates 47 out of 105 e. coli isolates (44.8%) contained blactx m gene and 37 isolates (35.2%) harbored blatem gene. also 17 isolates (16.2%) contained both blactx m and blatem genes simultaneously. according to the results blashv gene was not detected among the studied isolates. also no significant difference was seen in distribution of esbl genes among the studied regions. conclusions: the results of this study indicate that water buffalo gastrointestinal e. coli is reservoir for esbls especially ctx m and tem types and this should be considered in terms of public health and the transfer of resistance genes to pathogenic bacteria.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023